Transcriptional control of yeast plasma membrane H(+)-ATPase by glucose. Cloning and characterization of a new gene involved in this regulation

J Biol Chem. 1994 Jul 8;269(27):18076-82.


The expression of the ATPase gene (PMA1) is regulated by glucose (Rao, R., Drummond-Barbosa, D., and Slayman, C. W. (1993) Yeast 9, 1075-1084) and by the TUF/RAP1/GRF1 transcription factor (Capieaux, E., Vignais, M.-L., Sentenac, A., and Goffeau, A. (1989) J. Biol. Chem. 264, 7437-7446). In this work, we describe the isolation of mutations on seven genes that affect the levels of ATPase. One of these genes (APA1) was cloned by complementation and shown to encode a protein with six putative transmembrane stretches. Expression of APA1 gene is regulated by the carbon source and requires the protein GCR1. Deletion of APA1 causes a defective regulation of the PMA1 expression by glucose but has not noticeable effect on the expression of other TUF-regulated genes. Nevertheless the expression of glucose-repressible HXT3 and SNF3 genes is significantly reduced. These results suggest a model in which APA1 acts on a glucose-signaling pathway that controls the expression of several genes that are transcriptionally regulated by glucose.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cell Membrane / enzymology
  • Cloning, Molecular
  • DNA, Fungal
  • Gene Expression Regulation, Fungal*
  • Genes, Fungal
  • Genes, Regulator
  • Genetic Complementation Test
  • Glucose / physiology*
  • Molecular Sequence Data
  • Monosaccharide Transport Proteins / genetics
  • Monosaccharide Transport Proteins / metabolism
  • Mutation
  • Proton-Translocating ATPases / genetics*
  • Proton-Translocating ATPases / metabolism
  • Saccharomyces cerevisiae / enzymology
  • Saccharomyces cerevisiae / genetics*
  • Transcription, Genetic
  • Transformation, Genetic


  • DNA, Fungal
  • Monosaccharide Transport Proteins
  • Proton-Translocating ATPases
  • Glucose

Associated data

  • GENBANK/X78326