Mouse hepatitis virus gene 5b protein is a new virion envelope protein

Virology. 1994 Aug 1;202(2):1018-23. doi: 10.1006/viro.1994.1430.


Highly purified radiolabeled mouse hepatitis virus (MHV) A59 contained a previously overlooked protein which coelectrophoreses with the gene 5b product immunoprecipitated from infected cells. The gene 5b protein is post-translationally acylated. Rabbit antibody raised against a recombinant gene 5b protein expressed in Escherichia coli neutralized viral infectivity in the presence of complement, although not in the absence of complement. Immunofluorescent staining of MHV-infected cells with two anti-peptide antibodies revealed that the gene 5b product is membrane-associated and is transported to the cell surface, findings consistent with the prediction of a membrane-spanning segment in the gene 5b polypeptide. These results suggest strongly that the gene 5b polypeptide represents a new MHV virion envelope protein which is homologous to the TGEV ORF 4 and IBV 3c proteins.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antigens, Viral / chemistry
  • Antigens, Viral / metabolism
  • Cell Membrane / metabolism
  • Murine hepatitis virus / chemistry*
  • Murine hepatitis virus / immunology
  • Murine hepatitis virus / metabolism
  • Neutralization Tests
  • Protein Processing, Post-Translational
  • Viral Envelope Proteins / chemistry*
  • Viral Envelope Proteins / immunology
  • Viral Envelope Proteins / metabolism*
  • Virion / chemistry*


  • Antigens, Viral
  • Viral Envelope Proteins
  • gene 5b protein, hepatitis virus