Anti-CD31 delays platelet adhesion/aggregation at sites of endothelial injury in mouse cerebral arterioles

Am J Pathol. 1994 Jul;145(1):33-6.


The arterioles on the surface of the mouse brain (pial arterioles) were observed by in vivo microscopy. A focus of minor endothelial damage was produced in a single pial arteriole in each mouse by briefly exposing the site to a helium neon laser after an intravenous injection of Evans blue. Mice were injected 10 minutes before injury with a monoclonal antibody (MAb) to mouse CD31, also known as platelet endothelial cell adhesion molecule. This treatment doubled (P < .01) the time required for the laser to produce a recognizable platelet aggregate. In additional experiments, an MAb to mouse CD61 and an MAb to mouse intercellular adhesion molecule 1 had no effect. The data support previous observations indicating that platelet adhesion/aggregation in this model is induced by endothelial injury without exposure of basal lamina. The data are consistent with the hypothesis that the endothelial injury exposes or activates a platelet endothelial cell adhesion molecule on the endothelium which is blocked by the MAb directed against CD31. This may be the first demonstration of an effect of an anti-platelet endothelial cell adhesion molecule on platelet endothelial cell adhesion molecule on platelet adhesion/aggregation in vivo.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / pharmacology*
  • Antigens, Differentiation, Myelomonocytic / immunology*
  • Arterioles / drug effects
  • Brain / blood supply
  • Brain / drug effects
  • Cell Adhesion Molecules / immunology*
  • Endothelium, Vascular / drug effects
  • Endothelium, Vascular / injuries*
  • Mice
  • Platelet Adhesiveness / drug effects*
  • Platelet Aggregation / drug effects*
  • Platelet Endothelial Cell Adhesion Molecule-1


  • Antibodies, Monoclonal
  • Antigens, Differentiation, Myelomonocytic
  • Cell Adhesion Molecules
  • Platelet Endothelial Cell Adhesion Molecule-1