Keratocyte loss and repopulation of anterior corneal stroma after de-epithelialization

Arch Ophthalmol. 1994 Jul;112(7):973-6. doi: 10.1001/archopht.1994.01090190121031.


Objective: To quantitate corneal keratocyte loss and repopulation of anterior stroma after de-epithelialization.

Methods: Fourteen white New Zealand rabbits, each weighing 2.3 to 3.2 kg, were divided into seven groups. Each rabbit underwent a bilateral 6-mm mechanical deepithelialization procedure. The rabbits were killed two at a time after 1, 3, 6, 8, 10, 14, and 28 days. The corneas were labeled for mitosis using 5-bromo-2-deoxyuridine and stained for keratocyte quantification. Three untreated rabbits were added as controls.

Results: Corneas that were not operated on showed a higher density of keratocyte nuclei within the anterior corneal stroma than in the posterior stroma. Following de-epithelialization, there was a decrease in the ratio of anterior-posterior keratocytes, with maximal decrease on the third postoperative day. Keratocyte repopulation was completed by day 14. Mitotic activity was seen on days 1, 3, and 6 in the anterior half of the de-epithelialized stroma.

Conclusions: Anterior stromal keratocytes are lost after epithelial removal, but repopulation is complete within 2 weeks. These findings demonstrate a loss of keratocytes and their recovery after corneal epithelial removal, as well as an interaction between corneal epithelium and stroma in rabbits.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bromodeoxyuridine / metabolism
  • Cell Death
  • Cell Division
  • Cell Movement
  • Cornea / cytology
  • Cornea / physiology*
  • Corneal Stroma / cytology*
  • DNA / biosynthesis
  • Epithelial Cells
  • Epithelium / physiology
  • Mitosis
  • Rabbits
  • Wound Healing


  • DNA
  • Bromodeoxyuridine