The RAD6 DNA repair pathway in Saccharomyces cerevisiae: what does it do, and how does it do it?

Bioessays. 1994 Apr;16(4):253-8. doi: 10.1002/bies.950160408.


The RAD6 pathway of budding yeast, Saccharomyces cerevisiae, is responsible for a substantial fraction of this organism's resistance to DNA damage, and also for induced mutagenesis. The pathway appears to incorporate two different recovery processes, both regulated by RAD6. The error-prone recovery process accounts for only a small amount of RAD6-dependent resistance, but probably all induced mutagenesis. The underlying mechanism for error-prone recovery is very likely to be translesion synthesis. The error-free recovery process accounts for most of RAD6-dependent resistance, but its mechanism is less clear; it may entail error-free bypass by template switching and/or DNA gap filling by recombination. RAD6 regulates these activities by ubiquitinating target proteins, but the identities of these target proteins, and the roles they play in error-free and error-prone recovery, have not yet been established.

Publication types

  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • DNA Damage
  • DNA Repair*
  • DNA, Fungal / genetics*
  • DNA, Fungal / metabolism
  • DNA, Fungal / radiation effects
  • Fungal Proteins / genetics
  • Fungal Proteins / metabolism*
  • Genes, Fungal
  • Ligases / genetics
  • Ligases / physiology*
  • Models, Biological
  • Mutation
  • Radiation Tolerance / genetics
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / physiology*
  • Saccharomyces cerevisiae / radiation effects
  • Saccharomyces cerevisiae Proteins*
  • Ubiquitin-Conjugating Enzymes


  • DNA, Fungal
  • Fungal Proteins
  • Saccharomyces cerevisiae Proteins
  • RAD6 protein, S cerevisiae
  • Ubiquitin-Conjugating Enzymes
  • Ligases