Evaluation of chromatographic methods for the determination of nifedipine in human serum

J Chromatogr A. 1994 May 13;668(2):469-73. doi: 10.1016/0021-9673(94)80141-X.


Gas-liquid chromatography and high-performance liquid chromatography were compared for the identification and determination of nifedipine in biological samples and the elaboration of the optimum liquid-liquid extraction procedure. The determination limits were 2 and 10 ng/ml, respectively, and the detection limits were 1 and 5 ng/ml, respectively. The calibration graphs were linear in the ranges 2-300 and 10-500 ng/ml, respectively. Recoveries based on three different concentrations were 88.7-95.8% and 93.7-104.2%, respectively. Both methods are sensitive, specific and reproducible enough for pharmacokinetic studies and therapeutic drug monitoring.

Publication types

  • Comparative Study

MeSH terms

  • Chromatography, Gas / methods*
  • Chromatography, Gas / standards
  • Chromatography, High Pressure Liquid / methods*
  • Chromatography, High Pressure Liquid / standards
  • Humans
  • Nifedipine / blood*


  • Nifedipine