Plasmalemmal dihydropyridine receptors modify the function of subplasmalemmal inositol 1,4,5-trisphosphate receptors: a hypothesis

Cell Calcium. 1994 May;15(5):431-7. doi: 10.1016/0143-4160(94)90018-3.

Abstract

Experimental observations on rat glomerulosa cells inspired a model which postulates that plasmalemmal dihydropyridine receptors are in juxtaposition and interaction with inositol 1,4,5-trisphosphate receptors in subplasmalemmal calciosomes. Activation of dihydropyridine receptors promotes the Ca2+ releasing effect of inositol 1,4,5-trisphosphate. The most important observations compatible with the model are the following: (1) angiotensin II does not influence Ca2+ influx during the peak phase of Ca2+ signal; (2) dihydropyridine drugs modify the initial peak of the Ca2+ signal induced by angiotensin II; (3) inhibitors of the dihydropyridine receptor reduce the initial Ca2+ signal also in the presence of 5 mM Ni2+, an inhibitor of voltage dependent Ca2+ influx; and (4) changes in extracellular K+ concentration within the physiological range also modify the cytoplasmic Ca2+ response to angiotensin II.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Angiotensin II / pharmacology
  • Animals
  • Calcium / physiology*
  • Calcium Channels / physiology*
  • Calcium Channels, L-Type
  • Cell Membrane / chemistry
  • Inositol 1,4,5-Trisphosphate / physiology*
  • Inositol 1,4,5-Trisphosphate Receptors
  • Kidney Glomerulus / chemistry
  • Models, Biological
  • Muscle Proteins / physiology*
  • Nickel / pharmacology
  • Potassium / pharmacology
  • Rats
  • Receptors, Cytoplasmic and Nuclear / physiology*
  • Signal Transduction*

Substances

  • Calcium Channels
  • Calcium Channels, L-Type
  • Inositol 1,4,5-Trisphosphate Receptors
  • Muscle Proteins
  • Receptors, Cytoplasmic and Nuclear
  • Angiotensin II
  • nickel chloride
  • Nickel
  • Inositol 1,4,5-Trisphosphate
  • Potassium
  • Calcium