The Activities of Two Ets-related Transcription Factors Required for Drosophila Eye Development Are Modulated by the Ras/MAPK Pathway

Cell. 1994 Jul 15;78(1):137-47. doi: 10.1016/0092-8674(94)90580-0.

Abstract

We show that the activities of two Ets-related transcription factors required for normal eye development in Drosophila, pointed and yan, are regulated by the Ras1/MAPK pathway. The pointed gene codes for two related proteins, and we show that one form is a constitutive activator of transcription, while the activity of the other form is stimulated by the Ras1/MAPK pathway. Mutation of the single consensus MAPK phosphorylation site in the second form abrogates this responsiveness. yan is a negative regulator of photoreceptor determination, and genetic data suggest that it acts as an antagonist of Ras1. We demonstrate that yan can repress transcription and that this repression activity is negatively regulated by the Ras1/MAPK signal, most likely through direct phosphorylation of yan by MAPK.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Calcium-Calmodulin-Dependent Protein Kinases / genetics
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / physiology*
  • Drosophila / genetics*
  • Drosophila Proteins*
  • Extracellular Signal-Regulated MAP Kinases*
  • Eye / growth & development
  • Eye Proteins / genetics
  • Eye Proteins / physiology*
  • Gene Expression Regulation
  • Genes, Insect / genetics
  • Genes, ras / genetics
  • Models, Biological
  • Morphogenesis
  • Nerve Tissue Proteins
  • Phenotype
  • Photoreceptor Cells, Invertebrate / growth & development
  • Proto-Oncogene Proteins / genetics
  • Proto-Oncogene Proteins / physiology*
  • Proto-Oncogene Proteins p21(ras) / genetics
  • Repressor Proteins*
  • Signal Transduction / physiology*
  • Transcription Factors / genetics
  • Transcription Factors / physiology*
  • Transcription, Genetic / genetics

Substances

  • AOP protein, Drosophila
  • DNA-Binding Proteins
  • Drosophila Proteins
  • Eye Proteins
  • Nerve Tissue Proteins
  • Proto-Oncogene Proteins
  • Repressor Proteins
  • Transcription Factors
  • pnt protein, Drosophila
  • Calcium-Calmodulin-Dependent Protein Kinases
  • Extracellular Signal-Regulated MAP Kinases
  • rl protein, Drosophila
  • Proto-Oncogene Proteins p21(ras)