Negative regulation of the growth-promoting transcription factor E2F-1 by a stably bound cyclin A-dependent protein kinase

Cell. 1994 Jul 15;78(1):161-72. doi: 10.1016/0092-8674(94)90582-7.


Cyclin A-kinase, an enzyme required for coordinating S phase progression, forms stable in vivo complexes with E2F-1, a growth-promoting transcription factor, which binds to the retinoblastoma gene product and is involved in the timely activation of genes whose products contribute to G1 exit and S phase traversal. Complex formation results in a negative biochemical effect of cyclin A-kinase: the shut-off of E2F-1-dependent DNA binding function in S/G2. Thus, specific and timely cell cycle-dependent interactions of E2F-1 with proteins that inhibit its function (i.e., RB during G1 and cyclin A-kinase during S/G2) may contribute to the periodicity of expression of certain E2F-1-responsive genes at the G1/S transition.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 3T3 Cells
  • Amino Acid Sequence
  • Animals
  • Carrier Proteins*
  • Cell Cycle / physiology*
  • Cell Cycle Proteins*
  • Cell Line
  • Cyclins / genetics
  • Cyclins / metabolism*
  • DNA / metabolism
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism
  • E2F Transcription Factors
  • E2F1 Transcription Factor
  • Gene Expression Regulation
  • Humans
  • Mice
  • Molecular Sequence Data
  • Precipitin Tests
  • Protein Binding
  • Protein Kinases / metabolism*
  • Protein Structure, Tertiary
  • Recombinant Fusion Proteins / metabolism
  • Retinoblastoma Protein / metabolism
  • Retinoblastoma-Binding Protein 1
  • Transcription Factor DP1
  • Transcription Factors / genetics
  • Transcription Factors / metabolism*


  • Arid4a protein, mouse
  • Carrier Proteins
  • Cell Cycle Proteins
  • Cyclins
  • DNA-Binding Proteins
  • E2F Transcription Factors
  • E2F1 Transcription Factor
  • E2F1 protein, human
  • E2f1 protein, mouse
  • Recombinant Fusion Proteins
  • Retinoblastoma Protein
  • Retinoblastoma-Binding Protein 1
  • Transcription Factor DP1
  • Transcription Factors
  • DNA
  • Protein Kinases