Polymorphism of tumour necrosis factor-alpha (TNF-alpha) at position -308 in relation to ankylosing spondylitis

Clin Exp Immunol. 1994 Jul;97(1):45-7. doi: 10.1111/j.1365-2249.1994.tb06577.x.


In addition to HLA-B27, other genetic factors are thought to be involved in the pathogenesis of ankylosing spondylitis (AS). Because of the localization, in the proximity of the HLA-B locus, and the biological activities of TNF-alpha, we investigated the association between AS and a single base polymorphism located at position -308 of the TNF-alpha gene. An allele-specific polymerase chain reaction was developed to monitor this polymorphism. The frequency of the TNF-alpha alleles was determined in 66 AS patients and 37 healthy controls. The TNF-alpha allele frequency was not significantly different between AS patients and controls.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles
  • Base Sequence
  • DNA Primers / genetics
  • Gene Frequency
  • HLA-B27 Antigen / genetics
  • Humans
  • Molecular Sequence Data
  • Polymorphism, Genetic*
  • Spondylitis, Ankylosing / genetics*
  • Spondylitis, Ankylosing / immunology*
  • Tumor Necrosis Factor-alpha / genetics*


  • DNA Primers
  • HLA-B27 Antigen
  • Tumor Necrosis Factor-alpha