Myelin membrane biogenesis by oligodendrocytes. Developmental regulation of low molecular weight GTP-binding proteins

FEBS Lett. 1994 Jun 27;347(2-3):273-8. doi: 10.1016/0014-5793(94)00562-1.


Oligodendrocytes synthesize dramatic amounts of myelin membrane. We hypothesized that this requires unique aspects of vesicular trafficking. Specific stages of the oligodendrocyte lineage were assayed for low molecular weight GTP-binding proteins implicated in the regulation of vesicular transport pathway (two dimensional gel electrophoresis, [alpha-32P]GTP overlay). Consistent with the hypothesis, as oligodendrocytes differentiate from early progenitors to mature myelin-producing cells, > or = 12 small GTP-binding proteins become up-regulated. Myelin membrane also has a complex pattern of GTP-binding proteins. Several of these proteins may be specific to oligodendrocytes, suggesting that oligodendrocytes may utilize cell-type specific GTP-binding proteins for biogenesis and maintenance of the myelin membrane.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cell Differentiation
  • Cells, Cultured
  • Exocytosis
  • GTP-Binding Proteins / physiology*
  • Guanosine Triphosphate / metabolism
  • Immunoblotting
  • Myelin Sheath / physiology*
  • Oligodendroglia / cytology
  • Oligodendroglia / physiology*
  • Rats
  • Stem Cells / cytology


  • Guanosine Triphosphate
  • GTP-Binding Proteins