Molecular cloning of a bovine MSH receptor which is highly expressed in the testis

FEBS Lett. 1994 Jul 18;348(3):268-72. doi: 10.1016/0014-5793(94)00619-9.

Abstract

A novel G protein-coupled receptor (BDF3) was isolated from bovine genomic DNA by a combined approach of polymerase chain reaction (PCR) and hybridization techniques. The predicted amino acid sequence is 317 amino acids in length and displays 80% homology to the human alpha-MSH receptor MC1. Stably transfected into CHO-K1 cells, BDF3 mediates an increase of intracellular cAMP-levels following incubation with NLE-alpha-MSH, a potent alpha-MSH analog. The stimulation with ACTH1-10 is only moderate and gamma-MSH is ineffective. Northern blot analysis of bovine tissues revealed that the BDF3 gene is highly expressed in the testis as a single 2.3 kb mRNA species, suggesting an involvement of the BDF3 receptor in spermatogenesis.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • CHO Cells
  • Cattle
  • Cloning, Molecular*
  • Cricetinae
  • DNA / chemistry
  • GTP-Binding Proteins / metabolism
  • Gene Expression*
  • Humans
  • Male
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Polymerase Chain Reaction
  • Receptors, Pituitary Hormone / chemistry
  • Receptors, Pituitary Hormone / genetics*
  • Sequence Homology
  • Testis / metabolism*
  • Transfection

Substances

  • Receptors, Pituitary Hormone
  • MSH receptor
  • DNA
  • GTP-Binding Proteins

Associated data

  • GENBANK/S71017