Abstract
A soluble, periplasmic protein was isolated from Escherichia coli cells by chromatography on columns of aspartate-coupled Sepharose 4B. This protein has a molecular weight of about 15,000 and, as judged by competition experiments, binds the three dicarboxylic acids, succinate, malate, and fumarate and, addition, the monocarboxylic acid D-lactate. The periplasmic protein seems to be missing from some mutants of E. coli (designated cbt) which are incapable of transporting succinate in whole cells.
MeSH terms
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Bacterial Proteins / isolation & purification*
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Bacterial Proteins / metabolism
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Biological Transport, Active
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Carboxylic Acids / pharmacology
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Chromatography, Affinity
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Dicarboxylic Acids / metabolism*
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Edetic Acid / pharmacology
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Escherichia coli / drug effects
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Escherichia coli / metabolism*
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Fumarates / metabolism
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Kinetics
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Lactates / metabolism
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Magnesium / pharmacology
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Malates / metabolism
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Molecular Weight
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Receptors, Drug*
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Solubility
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Structure-Activity Relationship
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Succinates / metabolism
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Sulfhydryl Reagents / pharmacology
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Zinc / pharmacology
Substances
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Bacterial Proteins
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Carboxylic Acids
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Dicarboxylic Acids
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Fumarates
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Lactates
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Malates
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Receptors, Drug
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Succinates
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Sulfhydryl Reagents
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Edetic Acid
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Magnesium
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Zinc