Effects of lipoxin A4 on chemotaxis and degranulation of human eosinophils stimulated by platelet-activating factor and N-formyl-L-methionyl-L-leucyl-L-phenylalanine

Allergy. 1994 Apr;49(4):230-4. doi: 10.1111/j.1398-9995.1994.tb02654.x.


Lipoxins are trihydroxytetraene metabolites derived through a double lipoxygenation of arachidonic acid. Lipoxin A4 (LXA4) was prepared by total chemical synthesis, and its capacity to modulate eosinophil migration has been evaluated. LXA4 is a weak and partial chemotactic agent; at 10(-6) M, it achieved about 20% of the response of 10(-6) M platelet-activating factor (PAF). Preincubation of eosinophils with increasing doses of LXA4 (10(-10)-10(-5) M) resulted in a concentration-dependent inhibition of cell migration induced by 10(-6) M formyl-methionyl-leucyl-phenylalanine (FMLP) and 10(-6) M PAF. The concentration of LXA4 which produced 50% inhibition (IC50) of eosinophil migration was approximately 10(-6) M. LXA4 (10(-10)-10(-6) M) did not elicit ECP release or modulate ECP release induced by 10(-6) M FMLP. LXA4 may have antiallergic properties in preventing eosinophilic migration.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blood Proteins / metabolism
  • Cell Degranulation / drug effects*
  • Chemotaxis, Leukocyte / drug effects*
  • Eosinophil Granule Proteins
  • Eosinophils / drug effects
  • Eosinophils / physiology*
  • Humans
  • Hydroxyeicosatetraenoic Acids / pharmacology*
  • Lipoxins*
  • N-Formylmethionine Leucyl-Phenylalanine / pharmacology*
  • Platelet Activating Factor / pharmacology*
  • Ribonucleases*


  • Blood Proteins
  • Eosinophil Granule Proteins
  • Hydroxyeicosatetraenoic Acids
  • Lipoxins
  • Platelet Activating Factor
  • lipoxin A4
  • N-Formylmethionine Leucyl-Phenylalanine
  • Ribonucleases