Abstract
Interleukin 10 (IL-10) is produced by TH2 lymphocytes and regulates both lymphoid and myeloid cells. In the present study we demonstrate that IL-10 is expressed and produced spontaneously in the peripheral blood mononuclear cells (PBMCs) of all HIV-1 infected individuals tested, 3 of 19 cases of HIV-negative lymphoma and none of five healthy controls. IL-10 mRNA was detectable in both monocytes/macrophages and T lymphocytes isolated from PBMCs of HIV infected patients. We have also shown that infection of promonocytic (U937) and T (H9) cell lines with HIV stimulates IL-10 secretion. Furthermore, a T cell line (H9) stably transfected with a HIV tat expression-vector secreted higher levels of IL-10. We have also demonstrated that rhIL-10 inhibited HIV-1 replication in infected monocytes and PBMCs in a dose dependent manner. IL-10 may thus participate in long latency between HIV-1 infection and development of AIDS.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Acquired Immunodeficiency Syndrome / blood
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Acquired Immunodeficiency Syndrome / immunology*
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B-Lymphocytes / immunology*
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B-Lymphocytes / metabolism
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Base Sequence
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Cell Line
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DNA Primers
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Gene Expression
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Gene Products, tat / metabolism*
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Genes, tat
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HIV Seronegativity
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HIV-1 / drug effects
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HIV-1 / physiology*
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Humans
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Interleukin-10 / biosynthesis
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Interleukin-10 / pharmacology*
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Interleukin-10 / physiology*
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Lymphoma / blood
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Lymphoma / immunology
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Macrophages / immunology
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Molecular Sequence Data
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Monocytes / immunology
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Monocytes / metabolism
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Polymerase Chain Reaction / methods
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RNA, Messenger / analysis
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RNA, Messenger / biosynthesis
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Reference Values
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T-Lymphocytes / immunology*
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T-Lymphocytes / metabolism
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Transfection
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Virus Replication / drug effects
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Virus Replication / physiology*
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tat Gene Products, Human Immunodeficiency Virus
Substances
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DNA Primers
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Gene Products, tat
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RNA, Messenger
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tat Gene Products, Human Immunodeficiency Virus
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Interleukin-10