Rapid typing of group A streptococci by the use of DNA amplification and non-radioactive allele-specific oligonucleotide probes

FEMS Microbiol Lett. 1994 Jun 1;119(1-2):19-25. doi: 10.1111/j.1574-6968.1994.tb06861.x.


Because of the allelic variations within the M protein gene (emm gene) of group A streptococci, reliable typing of this important human pathogen can be accomplished by the use of emm gene-specific oligonucleotide probes. Two technical modifications (a reverse dot blot and a reverse line blot hybridization assay) of a novel approach for the type-specific identification of emm genes have been developed. Both procedures involved amplification of an emm gene by polymerase chain reaction. The non-radioactively labeled amplicon was subsequently hybridized to a membrane carrying an array of immobilized emm gene-specific oligonucleotide probes, thus allowing the simultaneous analysis of the gene polymorphism in a single hybridization reaction. The feasibility of these rapid and easy to perform methods was shown for the unequivocal identification of reference strains and clinical isolates belonging to 16 different M serotypes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles
  • Antigens, Bacterial*
  • Bacterial Outer Membrane Proteins*
  • Bacterial Proteins / genetics
  • Bacterial Typing Techniques*
  • Base Sequence
  • Carrier Proteins*
  • Molecular Sequence Data
  • Oligonucleotide Probes*
  • Polymerase Chain Reaction*
  • Streptococcus pyogenes / classification*
  • Streptococcus pyogenes / genetics


  • Antigens, Bacterial
  • Bacterial Outer Membrane Proteins
  • Bacterial Proteins
  • Carrier Proteins
  • Oligonucleotide Probes
  • streptococcal M protein