Localization of transcription factor GATA-4 to regions of the mouse embryo involved in cardiac development

Dev Biol. 1994 Aug;164(2):361-73. doi: 10.1006/dbio.1994.1206.


To clarify the role of transcription factor GATA-4 in mammalian development, we have determined the pattern of expression of GATA-4 in early postimplantation mouse embryos. Using in situ hybridization and immunohistochemistry, we find that GATA-4 RNA and protein are expressed in cells associated with heart development. Intraembryonic expression of GATA-4 RNA is first apparent in coelomic epithelial cells of the primitive streak embryo (approximately 7.0-7.5 days postcoitum). During formation and bending of the heart tube (approximately 8 days postcoitum), GATA-4 RNA and protein are expressed in endocardium, myocardium, and embryonic structures containing precardiac mesoderm such as the septum transversum and intraembryonic coelomic epithelium. By the onset of cardiac septation (approximately 9 days postcoitum), abundant GATA-4 RNA expression is evident in endocardium, endocardial cushion tissue, and myocardium. Expression of GATA-4 by the myocardium continues through gestation and after birth. The temporal and spacial patterns of GATA-4 expression support a role for this factor in the regulation of cardiac differentiation, analogous to the established role of transcription factor GATA-1 in the regulation of hematopoiesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • DNA-Binding Proteins / biosynthesis*
  • Female
  • GATA4 Transcription Factor
  • Heart / embryology*
  • Immunohistochemistry
  • In Situ Hybridization
  • Male
  • Mice
  • Morphogenesis
  • Myocardium / metabolism*
  • Transcription Factors / biosynthesis*


  • DNA-Binding Proteins
  • GATA4 Transcription Factor
  • Transcription Factors