Isolation of integral membrane proteins by phase partitioning with Triton X-114

Methods Enzymol. 1994;228:182-93. doi: 10.1016/0076-6879(94)28019-3.
No abstract available

MeSH terms

  • Antigens, Bacterial / analysis
  • Antigens, Bacterial / isolation & purification
  • Bacterial Proteins / analysis
  • Bacterial Proteins / isolation & purification*
  • Chromatography / methods
  • Chromatography, Gel
  • Detergents*
  • Electrophoresis, Polyacrylamide Gel / methods
  • Humans
  • Immunoblotting
  • Indicators and Reagents
  • Membrane Proteins / analysis
  • Membrane Proteins / isolation & purification*
  • Polyethylene Glycols*
  • Treponema pallidum / chemistry

Substances

  • Antigens, Bacterial
  • Bacterial Proteins
  • Detergents
  • Indicators and Reagents
  • Membrane Proteins
  • Polyethylene Glycols
  • Nonidet P-40