Effect of beta-alanyl-L-histidinato zinc on differentiation of osteoblastic MC3T3-E1 cells: increases in alkaline phosphatase activity and protein concentration

Mol Cell Biochem. 1994 Feb 9;131(1):19-24. doi: 10.1007/BF01075720.


The effect of beta-alanyl-L-histidinato zinc (AHZ) on bone cell function was investigated in osteoblastic MC3T3-E1 cells. Cells were cultured for 3 days at 37 degrees C in a CO2 incubator in plastic dishes containing alpha-modified minimum essential medium supplemented with 10% fetal bovine serum. After the cultures, the medium was exchanged for that containing 0.1% bovine serum albumin plus AHZ (10(-7)-10(-5) M) or other reagents, and the cells were cultured further for appropriate periods of time. The presence of AHZ (10(-7)-10(-5) M) produced a remarkable increase of alkaline phosphatase activity and protein concentration in osteoblastic cells. Thus increase were seen with the prolonged cultivation (12-21 days). With the culture of 1, 3 and 12 days, the effect of AHZ (10(-6) M) to increase alkaline phosphatase activity and protein concentration was more intensive than the effect of zinc sulfate (10(-6) M). The AHZ effects were completely abolished by the presence of cycloheximide (10(-6) M), indicating that AHZ stimulates protein synthesis in the cells. The present study suggests that AHZ has a stimulatory effect on cell differentiation, and that this effect is partly involved on protein synthesis in osteoblastic cells.

Publication types

  • Comparative Study

MeSH terms

  • Alkaline Phosphatase / drug effects*
  • Alkaline Phosphatase / metabolism
  • Carnosine* / analogs & derivatives*
  • Cell Differentiation / drug effects
  • Cell Division / drug effects
  • Cell Line
  • Cycloheximide / pharmacology
  • Dipeptides / pharmacology*
  • Organometallic Compounds / pharmacology*
  • Osteoblasts / cytology
  • Osteoblasts / drug effects*
  • Proteins / drug effects*
  • Proteins / metabolism
  • Zinc / pharmacology*
  • Zinc Compounds


  • Dipeptides
  • Organometallic Compounds
  • Proteins
  • Zinc Compounds
  • polaprezinc
  • Carnosine
  • Cycloheximide
  • Alkaline Phosphatase
  • Zinc