The ETS domain protein pointed-P2 is a target of MAP kinase in the sevenless signal transduction pathway

Nature. 1994 Aug 4;370(6488):386-9. doi: 10.1038/370386a0.


The fate of the R7 photoreceptor cell in the developing eye of Drosophila is controlled by the Sevenless (Sev) receptor tyrosine kinase. Sev activates a highly conserved signal transduction cascade involving the proteins Ras1 and Raf and the Rolled/mitogen-activated protein (Rl/MAP) kinase. Here we show that the ETS domain protein encoded by the P2 transcript of the pointed (pnt) gene is a nuclear target of this signalling cascade which acts downstream of Rl/MAP kinase. The PntP2 protein is phosphorylated by Rl/MAP kinase in vitro at a single site and this site is required for its function in vivo. Furthermore, we present genetic and biochemical data suggesting that MAP kinase controls neural development through phosphorylation of two antagonizing transcription factors of the ETS family, Yan and PntP2.

MeSH terms

  • Animals
  • Base Sequence
  • Calcium-Calmodulin-Dependent Protein Kinases / genetics
  • Calcium-Calmodulin-Dependent Protein Kinases / metabolism
  • DNA / metabolism
  • DNA-Binding Proteins / metabolism
  • Drosophila
  • Drosophila Proteins*
  • Extracellular Signal-Regulated MAP Kinases*
  • Eye / embryology
  • Eye / metabolism
  • Eye Proteins / genetics
  • Eye Proteins / metabolism*
  • Humans
  • Membrane Glycoproteins / genetics
  • Membrane Glycoproteins / metabolism*
  • Mice
  • Molecular Sequence Data
  • Nerve Tissue Proteins
  • Phosphorylation
  • Protein Binding
  • Proto-Oncogene Proteins / metabolism*
  • Rats
  • Receptor Protein-Tyrosine Kinases / metabolism*
  • Repressor Proteins*
  • Signal Transduction*
  • Transcription Factors / metabolism


  • AOP protein, Drosophila
  • DNA-Binding Proteins
  • Drosophila Proteins
  • Eye Proteins
  • Membrane Glycoproteins
  • Nerve Tissue Proteins
  • Proto-Oncogene Proteins
  • Repressor Proteins
  • Transcription Factors
  • pnt protein, Drosophila
  • DNA
  • Receptor Protein-Tyrosine Kinases
  • sev protein, Drosophila
  • Calcium-Calmodulin-Dependent Protein Kinases
  • Extracellular Signal-Regulated MAP Kinases
  • rl protein, Drosophila