cDNA cloning of human kidney pyruvate carboxylase

Biochem Biophys Res Commun. 1994 Jul 29;202(2):1009-14. doi: 10.1006/bbrc.1994.2029.


cDNA clones covering a total of 4017 bp were isolated corresponding to the full length of the message for human pyruvate carboxylase. The coding sequence consisted of a stretch of 3534 bp flanked by a 5' untranslated sequence of 82 bp and a 3' untranslated region of 389 bp excluding the poly-A tail. The translated amino acid sequence of 1178 residues contained consensus sequences for the covalent attachment for biotin, the binding of ATP and the binding of the substrate, pyruvate.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular*
  • Consensus Sequence
  • Conserved Sequence
  • DNA, Complementary / genetics*
  • Humans
  • Kidney / enzymology*
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Pyruvate Carboxylase / chemistry
  • Pyruvate Carboxylase / genetics*


  • DNA, Complementary
  • Pyruvate Carboxylase

Associated data

  • GENBANK/S72370