Endothelin-1 inhibits insulin-stimulated glucose uptake in isolated rat adipocytes

Biochem Biophys Res Commun. 1994 Jul 29;202(2):688-93. doi: 10.1006/bbrc.1994.1985.

Abstract

A single class of high affinity endothelin-1 (ET-1) binding sites with an apparent Kd of 350 pM and a binding capacity of 69,000 sites/cell was found in isolated rat adipocytes. Whereas ET-1 had no effect on basal glucose uptake, it inhibited insulin-stimulated glucose uptake in both a dose- and time-dependent manner. Insulin binding, however, was not altered. Thus, the present study demonstrates the presence of ET-1 receptor in isolated rat adipocytes and the inhibition of insulin-stimulated glucose uptake by ET-1 via a post-receptor mechanism.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipocytes / drug effects
  • Adipocytes / metabolism*
  • Animals
  • Binding, Competitive
  • Endothelins / metabolism
  • Endothelins / pharmacology*
  • Glucose / metabolism*
  • Iodine Radioisotopes
  • Male
  • Rats
  • Rats, Sprague-Dawley

Substances

  • Endothelins
  • Iodine Radioisotopes
  • Glucose