Hepatocyte growth factor/scatter factor modulates intestinal epithelial cell proliferation and migration

Biochem Biophys Res Commun. 1994 Jul 29;202(2):701-9. doi: 10.1006/bbrc.1994.1987.


Various peptide growth factors have been found to regulate epithelial cell function within the mucosal epithelium of the gastrointestinal tract. In this study hepatocyte growth factor/scatter factor (HGF/SF) was found to stimulate intestinal epithelial cell proliferation: 2.5-fold in the non-transformed rat small intestinal epithelial cell line IEC-6 and 1.9-fold in the human colon cancer-derived HT-29 cell line. In addition, HGF/SF enhanced epithelial cell restitution, the initial step involved in gastrointestinal wound healing, in an in vitro model. Migration of IEC-6 in wounded monolayers was enhanced up to 7-fold. Enhancement of restitution by HGF could be completely abrogated by addition of immunoneutralizing anti-TGF beta 1, indicating that this process is mediated through a TGF beta-dependent pathway. These findings suggest that HGF exerts functional effects on intestinal epithelial cell populations and may play a role in the morphogenesis of the gastrointestinal tract and its remodeling following injury.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cell Division*
  • Cell Line
  • Cell Movement*
  • Colonic Neoplasms
  • Epithelial Cells
  • Hepatocyte Growth Factor / pharmacology*
  • Humans
  • Intestines / cytology*
  • Rats
  • Recombinant Proteins / pharmacology
  • Transforming Growth Factor beta / pharmacology
  • Tumor Cells, Cultured


  • Recombinant Proteins
  • Transforming Growth Factor beta
  • Hepatocyte Growth Factor