Identification of the bphA and bphB genes of Pseudomonas sp. strains KKS102 involved in degradation of biphenyl and polychlorinated biphenyls

Biochem Biophys Res Commun. 1994 Jul 29;202(2):850-6. doi: 10.1006/bbrc.1994.2008.


The nucleotide sequence of the upstream region of the bphC gene from Pseudomonas sp. strain KKS102 was determined. Four genes were found in this region. Deduced amino acid sequences of the first, second, third and fourth genes showed significant homology with a large subunit of iron-sulfur protein, a small subunit of iron-sulfur protein, ferredoxin and dihydrodiol dehydrogenase, respectively, from other bacteria which degrade biphenyl/polychlorinated biphenyls, toluene and benzene. E. coli, in which the four genes, bphC and the gene for ferredoxin reductase from benzene degrading bacterium were expressed, was able to produce meta-cleavage compounds from chlorinated biphenyls. These results show that these gene products are functional in both biphenyl and polychlorinated biphenyls degradation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alcohol Oxidoreductases / chemistry
  • Alcohol Oxidoreductases / genetics*
  • Amino Acid Sequence
  • Base Sequence
  • Biphenyl Compounds / metabolism*
  • Escherichia coli / genetics
  • Ferredoxins / chemistry
  • Ferredoxins / genetics*
  • Gene Expression
  • Iron-Sulfur Proteins / chemistry
  • Iron-Sulfur Proteins / genetics*
  • Molecular Sequence Data
  • Open Reading Frames
  • Oxidoreductases / chemistry
  • Oxidoreductases / genetics
  • Oxidoreductases Acting on CH-CH Group Donors*
  • Polychlorinated Biphenyls / metabolism*
  • Pseudomonas / genetics*
  • Sequence Analysis, DNA


  • Biphenyl Compounds
  • Ferredoxins
  • Iron-Sulfur Proteins
  • Polychlorinated Biphenyls
  • Oxidoreductases
  • Alcohol Oxidoreductases
  • dihydrodiol dehydrogenases
  • ferredoxin-NAD+ reductase
  • Oxidoreductases Acting on CH-CH Group Donors
  • cis-1,2-dihydro-1,2-dihydroxynaphthalene dehydrogenase

Associated data

  • GENBANK/D17319