The hydrolysis of oestrone sulphate and dehydroepiandrosterone sulphate by human steroid sulphatase expressed in transfected COS-1 cells

J Steroid Biochem Mol Biol. 1994 Jul;50(1-2):101-4. doi: 10.1016/0960-0760(94)90177-5.


Whether the same or distinct steroid sulphatases (STS) are involved in the hydrolysis of alkyl and aryl steroid sulphates remains controversial. We have examined the ability of a placental steroid sulphatase to hydrolyse oestrone sulphate and/or dehydroepiandrosterone sulphate (DHA-S) by expressing the enzyme in COS-1 cells. Using either intact cells or broken cell preparations, the expressed sulphatase was found to hydrolyse both oestrone sulphate and DHA-S. The catalysis of oestrone sulphate and DHA-S by the expressed sulphatase was almost completely abolished by the steroid sulphatase inhibitor, oestrone-3-O-sulphamate. It is concluded that both alkyl and aryl steroid sulphates can be hydrolysed by the same steroid sulphatase.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Arylsulfatases / antagonists & inhibitors
  • Arylsulfatases / metabolism*
  • Catalysis
  • Cell Line
  • Chlorocebus aethiops
  • Dehydroepiandrosterone / analogs & derivatives*
  • Dehydroepiandrosterone / metabolism
  • Dehydroepiandrosterone Sulfate
  • Estrogens, Conjugated (USP) / metabolism*
  • Estrone / analogs & derivatives*
  • Estrone / metabolism
  • Estrone / pharmacology
  • Female
  • Humans
  • Hydrolysis
  • Placenta / enzymology
  • Steryl-Sulfatase
  • Transfection


  • Estrogens, Conjugated (USP)
  • estrone-3-O-sulfamate
  • Estrone
  • Dehydroepiandrosterone
  • Dehydroepiandrosterone Sulfate
  • Arylsulfatases
  • Steryl-Sulfatase
  • estrone sulfate