Is chicken green-sensitive cone visual pigment a rhodopsin-like pigment? A comparative study of the molecular properties between chicken green and rhodopsin

Biochemistry. 1994 Aug 9;33(31):9040-4. doi: 10.1021/bi00197a002.

Abstract

Chicken green is a visual pigment present in chicken green-sensitive cones and has an amino acid sequence more similar than any other cone visual pigments to the rod visual pigments, rhodopsins. Here we have investigated the molecular properties of chicken green and compared them with those of rhodopsin to elucidate whether or not chicken green is a rhodopsin-like pigment. While chicken green has a molecular extinction coefficient and a photosensitivity very similar to those of rhodopsin, it displays faster regeneration from 11-cis-retinal and opsin and faster formation and decay of the physiologically active meta II intermediate than rhodopsin. These differences correlate with the physiological difference between cones and rods. Thus in spite of the similarity in amino acid sequence, chicken green displays molecular properties required for a cone visual pigment that are clearly different from those of rhodopsin.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Avian Proteins
  • Chickens
  • Eye Proteins / chemistry*
  • Eye Proteins / isolation & purification
  • Light
  • Retinal Cone Photoreceptor Cells / physiology*
  • Retinal Pigments / chemistry*
  • Retinal Pigments / isolation & purification
  • Retinaldehyde
  • Rhodopsin / chemistry*
  • Rhodopsin / isolation & purification
  • Rod Opsins / chemistry
  • Rod Opsins / isolation & purification
  • Spectrophotometry

Substances

  • Avian Proteins
  • Eye Proteins
  • Retinal Pigments
  • Rod Opsins
  • RHO rhodopsin protein, chicken
  • iodopsin
  • Rhodopsin
  • Retinaldehyde