Isolation of a cytochrome P450 homologue preferentially expressed in developing inflorescences of Zea mays

Plant Mol Biol. 1994 Jun;25(3):343-53. doi: 10.1007/BF00043864.

Abstract

Four cDNA clones exhibiting preferential hybridization to transcripts present in developing maize tassels were isolated by differential screening. One of these cDNA clones hybridizes to transcripts detectable only in the shoot apex. The abundance of this transcript is significantly higher in developing inflorescence apices than in vegetative apices. DNA sequence analysis of a 2107 nucleotide cDNA clone corresponding to this transcript revealed that the transcript encodes a polypeptide of 547 amino acids, with a molecular mass of 58.4 kDa. This polypeptide shares significant sequence similarity with members of the cytochrome P450 monooxygenase gene superfamily, including the conserved C-terminal domains typical of the cytochrome P450 monooxygenases.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Cytochrome P-450 Enzyme System / genetics*
  • DNA, Complementary / genetics
  • Gene Library
  • Genes, Plant / genetics*
  • Molecular Sequence Data
  • Plant Proteins*
  • Reproduction
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Tissue Distribution
  • Zea mays / enzymology
  • Zea mays / genetics*
  • Zea mays / growth & development

Substances

  • DNA, Complementary
  • Plant Proteins
  • Cytochrome P-450 Enzyme System
  • CYP78A1 protein, Zea mays

Associated data

  • GENBANK/L23209