Differentiation of Cryptococcus neoformans serotypes A and D using creatinine dextrose bromothymol blue thymine medium

Br J Biomed Sci. 1994 Jun;51(2):100-3.

Abstract

A serotype differentiation of the encapsulated yeast Cryptococcus neoformans is described using creatinine dextrose bromothymol blue thymine (CDBT) medium. On CDBT medium C. neoformans serotype D grew as bright red colonies, turning the medium a bright orange after five days incubation at 28 degrees C. C. neoformans serotype A grew as pale colonies with no apparent colour effect on the medium. Serotypes B and C caused a slight greening of the medium. The reaction of the four serotypes of C. neoformans on CDBT medium is considered useful in the differentiation of the closely related serotype A and D.

MeSH terms

  • Bromthymol Blue*
  • Creatinine
  • Cryptococcus neoformans / classification*
  • Culture Media
  • Glucose
  • Hydrogen-Ion Concentration
  • Serotyping / methods*
  • Thymine

Substances

  • Culture Media
  • Creatinine
  • Glucose
  • Thymine
  • Bromthymol Blue