Structure of a Hexasaccharide Proximal to the Hydrophobic Region of Lipopolysaccharides Present in Bordetella Pertussis Endotoxin Preparations

Carbohydr Res. 1994 Jun 17;259(2):257-75. doi: 10.1016/0008-6215(94)84061-x.

Abstract

A branched-chain hexasaccharide containing 3-deoxy-D-manno-oct-2-ulosonic acid was released by detergent-promoted hydrolysis from Bordetella pertussis endotoxin preparations that were first dephosphorylated with aqueous HF and then treated with nitrous acid. Its structure (2) [Formula: See text] was determined by chemical and physical methods. This hexasaccharide is present in all four lipopolysaccharides that make up the B. pertussis strain 1414 (phase 1) endotoxin preparations analysed, and is situated near to the hydrophobic domains. An analogous structure reported previously (ref 7) is erroneous and should be disregarded.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bordetella pertussis / chemistry*
  • Carbohydrate Sequence
  • Deamination
  • Endotoxins / chemistry*
  • Gas Chromatography-Mass Spectrometry
  • Heptoses / analysis
  • Hexosamines / analysis
  • Hexoses / analysis
  • Hexuronic Acids / analysis
  • Hydrolysis
  • Lipopolysaccharides / chemistry*
  • Magnetic Resonance Spectroscopy
  • Molecular Sequence Data
  • Oligosaccharides / chemistry*
  • Oxidation-Reduction
  • Periodic Acid / chemistry
  • Phosphoproteins / chemistry
  • Sequence Analysis
  • Virulence Factors, Bordetella*

Substances

  • Endotoxins
  • Heptoses
  • Hexosamines
  • Hexoses
  • Hexuronic Acids
  • Lipopolysaccharides
  • Oligosaccharides
  • Phosphoproteins
  • Virulence Factors, Bordetella
  • pertussis endotoxin
  • Periodic Acid
  • metaperiodate