Expression of transforming growth factor-beta (TGF-beta) receptors, TGF-beta 1 and TGF-beta 2 production and autocrine growth control in osteosarcoma cells

Int J Cancer. 1994 Aug 1;58(3):440-5. doi: 10.1002/ijc.2910580323.

Abstract

Transforming growth factor-beta (TGF-beta) is a polypeptide with multiple physiological functions. Isoforms of this growth factor have important roles in control of the cell cycle, in regulation of cell-cell interactions and in growth and development. Malignant transformation has been shown to be associated with increased expression of TGF-beta. Since bone is the largest storage site and producer of TGF-beta, we speculated on the existence of an autocrine mechanism in osteosarcoma, a malignant bone tumor. Expression of TGF-beta cell surface receptors, effects on growth of TGF-beta and TGF-beta antibodies and production of 2 TGF-beta isoforms were studied in a panel of 7 osteosarcoma cell lines. In contrast to most previous reports on the effects of TGF-beta on osteosarcoma cell growth, we found a mitogenic effect of TGF-beta 1 in 4 of 7 osteosarcoma cell lines. Receptor profiles for TGF-beta were aberrant in 5 of the 7 cell lines tested, and production of TGF-beta 1 and TGF-beta 2 varied among cell lines. Addition of anti-TGF-beta antagonized the effects of endogenous TGF-beta. Our results suggest a potential role of TGF-beta in autocrine growth control of osteosarcoma cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies / pharmacology
  • Bone Neoplasms / metabolism*
  • Bone Neoplasms / pathology*
  • Bone Neoplasms / ultrastructure
  • Cell Division / drug effects
  • Cell Division / physiology
  • Culture Media
  • DNA, Neoplasm / biosynthesis
  • Humans
  • Isomerism
  • Osteosarcoma / metabolism*
  • Osteosarcoma / pathology*
  • Osteosarcoma / ultrastructure
  • Receptors, Transforming Growth Factor beta / biosynthesis
  • Receptors, Transforming Growth Factor beta / physiology*
  • Stimulation, Chemical
  • Transforming Growth Factor beta / biosynthesis
  • Transforming Growth Factor beta / pharmacology*
  • Transforming Growth Factor beta / physiology*
  • Tumor Cells, Cultured / drug effects

Substances

  • Antibodies
  • Culture Media
  • DNA, Neoplasm
  • Receptors, Transforming Growth Factor beta
  • Transforming Growth Factor beta