The combination of a transcutaneous microdialysis probe and continuous flow analysis was tested for continuous glucose monitoring in eight newborn infants who were fed intravenously. The probe was placed on the skin, which was first stripped with cellophane tape to increase the skin permeability. The skin was stripped until the transepidermal water loss reached values greater than 80 gm/m2/hr. Dialysate concentrations were monitored for 165 minutes while the blood glucose concentrations were manipulated by changing the infusion rate of glucose. Blood glucose concentration was linearly related to the dialysate concentration. Because the dialysate/blood glucose ratio varied among the infants, the dialysate concentration was calibrated to estimated blood values with a single-point and a multiple-point calibration method. The latter method yielded more accurate estimates of the blood values. We conclude that transcutaneous microdialysis may be used for glucose monitoring in newborn infants.