H2O2 induced hyperpolarization of pancreatic B-cells

Pflugers Arch. 1994 Apr;426(6):552-4. doi: 10.1007/BF00378534.


Conventional electrophysiology and the whole-cell patch-clamp technique have been applied to elucidate the effects of H2O2 on pancreatic B-cells of the mouse. In these cells, addition of 15 mmol/l glucose leads to depolarization and oscillation of the cell membrane potential. Subsequent addition of H2O2 (1 mmol/l) in the presence of glucose was followed by a marked and rapid hyperpolarization of the cell membrane with suppression of the electrical activity. Accordingly, in slow whole-cell patch-clamp experiments (with nystatin in the pipette solution) H2O2 induced a marked increase of cell membrane conductance. Tolbutamide, a blocker of K+ ATP channels, only partially blocked the effect of H2O2 even at high concentrations. The H2O2-induced, tolbutamide-insensitive current component, however, was largely abolished by a high concentration of TEA+ (80 mmol/l) or BaCl2 (10 mmol/l). It is concluded that in B-cells H2O2 stimulates a K+ current and that this effect leads to marked hyperpolarization and reversal of glucose-induced oscillations of cell membrane potential.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Barium Compounds / pharmacology
  • Cell Membrane / drug effects
  • Chlorides / pharmacology
  • Electrophysiology
  • Female
  • Glucose / pharmacology
  • Hydrogen Peroxide / antagonists & inhibitors
  • Hydrogen Peroxide / pharmacology*
  • In Vitro Techniques
  • Islets of Langerhans / drug effects
  • Islets of Langerhans / physiology*
  • Membrane Potentials / drug effects
  • Membrane Potentials / physiology
  • Mice
  • Potassium Channels / drug effects
  • Potassium Channels / metabolism
  • Quaternary Ammonium Compounds / pharmacology
  • Tolbutamide / pharmacology


  • Barium Compounds
  • Chlorides
  • Potassium Channels
  • Quaternary Ammonium Compounds
  • barium chloride
  • Tolbutamide
  • Hydrogen Peroxide
  • Glucose