Contacts of the globular domain of histone H5 and core histones with DNA in a "chromatosome"

J J Hayes; D Pruss; A P Wolffe

doi:10.1073/pnas.91.16.7817

Contacts of the globular domain of histone H5 and core histones with DNA in a "chromatosome"

Proc Natl Acad Sci U S A. 1994 Aug 2;91(16):7817-21. doi: 10.1073/pnas.91.16.7817.

Authors

J J Hayes¹, D Pruss, A P Wolffe

Affiliation

¹ Laboratory of Molecular Embryology, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892.

Abstract

The globular domain of histone H5 is found to asymmetrically associate with a nucleosome core including the Xenopus borealis somatic 5S RNA gene. Histones H2A and H2B are required for association of histone H5. Strong crosslinking of the globular domain of histone H5 to the 5S DNA in the nucleosome occurs at a single site to one side of the dyad axis. This site is also in contact with the core histones, and the interactions of the core histones with 5S DNA change as a result of association of the globular domain of histone H5. We discuss evidence for an allosteric change in core histone-5S DNA interactions following the association of the linker histone in the nucleosome.

MeSH terms

Animals
Cross-Linking Reagents
DNA, Ribosomal / chemistry*
Histones / chemistry*
Models, Structural
Molecular Conformation
Nucleosomes / chemistry*
RNA, Ribosomal, 5S / genetics
Xenopus

Substances

Cross-Linking Reagents
DNA, Ribosomal
Histones
Nucleosomes
RNA, Ribosomal, 5S