Cloning of a VLP-protein coding gene from a parasitoid wasp Venturia canescens

Arch Insect Biochem Physiol. 1994;26(2-3):137-45. doi: 10.1002/arch.940260207.

Abstract

Virus-like particles (VLPs) that are bound to the egg surface of a parasitic wasp Venturia canescens are void of nucleic acid and unable to infect host tissue but instead provide a passive protection against the host's immune recognition. To investigate evolutionary and functional properties of Venturia particles, we isolated cDNAs coding for a VLP-protein by screening an expression library of the wasp, using antibodies against purified VLPs. The corresponding coding DNA is present in the wasp genome as a single-copy gene.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Biological Evolution
  • Blotting, Western
  • Cloning, Molecular
  • DNA
  • DNA Transposable Elements*
  • Drosophila Proteins*
  • Exons
  • Gene Library
  • Molecular Sequence Data
  • Organ Specificity / genetics
  • Peptide Hydrolases*
  • Proteins / genetics*
  • Retroelements
  • Wasps / genetics*

Substances

  • DNA Transposable Elements
  • Drosophila Proteins
  • Proteins
  • Retroelements
  • DNA
  • Peptide Hydrolases
  • Copia protein, Drosophila

Associated data

  • GENBANK/S71574