This paper presents an overview of vitamin B6 and exercise, including the role that vitamin B6 plays in gluconeogenesis and glycogenolysis and changes in vitamin B6 metabolism during exercise. The dietary vitamin B6 intakes of athletes are also reviewed. Most studies report that male athletes have adequate dietary intakes of vitamin B6, whereas some females, especially those with low energy intakes, appear to have low vitamin B6 intakes. Few studies have assessed the vitamin B6 status of nonsupplementing athletes using the recommended status criteria. The role that vitamin B6 may play in attenuating the rise in plasma growth hormone observed during exercise is also reviewed. Finally, recommendations are given for further research in the area of vitamin B6 and exercise.