Potato (Solanum tuberosum) invertase-encoding cDNAs and their differential expression

Gene. 1994 Aug 5;145(2):211-4. doi: 10.1016/0378-1119(94)90007-8.


A full-length cDNA clone encoding a potato invertase (Inv) has been isolated. It is highly related (77% nucleotide identity) to a previously characterised potato cDNA clone encoding a putative extracellular Inv. These Inv genes encode a subfamily of apoplastic enzymes which are shown to be distinct, on the basis of sequence similarity, from the related subfamily of vacuolar enzymes. In order to differentiate between the expression of the two potato genes encoding apoplastic Inv, a single-stranded conformational polymorphism (SSCP) assay was developed for products generated by reverse transcription-polymerase chain reaction (RT-PCR) utilising primers designed to amplify both potato sequences. Using this approach, we have shown that these two identified Inv from potato are expressed in a tissue-specific and developmentally regulated manner.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • DNA, Complementary / genetics
  • Gene Expression
  • Genes, Plant / genetics*
  • Glycoside Hydrolases / genetics*
  • Molecular Sequence Data
  • Multigene Family / genetics*
  • Plastids
  • Polymerase Chain Reaction
  • Sequence Analysis, DNA
  • Solanum tuberosum / enzymology
  • Solanum tuberosum / genetics*
  • Tissue Distribution
  • beta-Fructofuranosidase


  • DNA, Complementary
  • Glycoside Hydrolases
  • beta-Fructofuranosidase

Associated data

  • GENBANK/Z22645