Cloning and expression analysis of the cDNA encoding rat Zn-alpha 2-glycoprotein

Gene. 1994 Aug 5;145(2):245-9. doi: 10.1016/0378-1119(94)90014-0.

Abstract

We report here the nucleotide (nt) sequence of a rat liver cDNA encoding Zn-alpha 2-glycoprotein (Zn-alpha 2-gp), a plasma protein with a high degree of sequence similarity to class-I major histocompatibility complex (MHC) antigens. The deduced amino acid (aa) sequence contains the coding information for 293 aa residues and shows 60% identity with the aa sequence of human Zn-alpha 2-gp and 35% identity with that corresponding to the extracellular domains of RT1, a rat class-I MHC antigen. Northern blot analysis showed that rat Zn-alpha 2-gp is expressed in liver, but not in a wide number of tissues, including prostate, mammary gland, kidney, intestine, lung, pancreas, ovary, uterus, thyroid, placenta, spleen, brain and heart.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • DNA, Complementary / genetics
  • Glycoproteins / genetics*
  • Histocompatibility Antigens Class I / genetics
  • Humans
  • Molecular Sequence Data
  • Rats
  • Seminal Plasma Proteins*
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Tissue Distribution

Substances

  • DNA, Complementary
  • Glycoproteins
  • Histocompatibility Antigens Class I
  • Seminal Plasma Proteins
  • Zn-alpha-2-glycoprotein

Associated data

  • GENBANK/X75309