Adenovirus myocarditis: retrospective diagnosis by gene amplification from formalin-fixed, paraffin-embedded tissues

Hum Pathol. 1994 Aug;25(8):831-4. doi: 10.1016/0046-8177(94)90255-0.


Understanding the pathogenesis of viral myocarditis is linked to the availability of sensitive assays to detect viruses in clinical material. Recent advances in molecular techniques permit direct detection of viral-specific nucleic acid in tissue samples. This report describes a protocol for DNA extraction and amplification of adenovirus genome from formalin-fixed, paraffin-embedded human tissues that detects as little as 10 copies of viral genome in a background of 0.5 micrograms of human DNA. This sensitive assay permitted the examination of archived tissues to establish a retrospective diagnosis of adenoviral myocarditis in two pediatric patients.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenovirus Infections, Human / diagnosis*
  • Adenovirus Infections, Human / microbiology
  • Adenoviruses, Human / genetics
  • Child
  • Child, Preschool
  • DNA, Viral / analysis*
  • Female
  • Humans
  • Male
  • Myocarditis / diagnosis*
  • Myocarditis / microbiology
  • Polymerase Chain Reaction*
  • Tissue Preservation*


  • DNA, Viral