Inhibitory effect of TGF-beta 2 in human aqueous humor on bovine lens epithelial cell proliferation

Invest Ophthalmol Vis Sci. 1994 Aug;35(9):3408-12.


Purpose: To determine whether transforming growth factor-beta 2 (TGF-beta 2) in human aqueous humor inhibits the proliferation of lens epithelial cells.

Methods: Bovine lens epithelial cells (BLECs) obtained from the central zone of the anterior capsule were cultured in complete medium (F-12 nutrient mixture supplemented with 5% fetal bovine serum). Human aqueous humor was obtained from 11 patients during cataract surgery. Medium was removed and replaced with medium containing various concentrations of porcine platelet-derived TGF-beta 2 or 10% human aqueous humor, with or without anti-TGF-beta 2 neutralizing antibody. The number of cells was counted at 96 hours.

Results: Porcine platelet-derived TGF-beta 2 inhibited BLEC proliferation in a dose-dependent manner. Anti-TGF-beta 2 antibody blocked the inhibitory effect of TGF-beta 2 on BLEC proliferation. The 10% human aqueous humor stimulated the proliferation of BLECs. Addition of anti-TGF-beta 2 antibody to the 10% human aqueous humor significantly enhanced this stimulatory effect on BLEC proliferation.

Conclusions: TGF-beta 2 in human aqueous humor inhibits the proliferation of lens epithelial cells in vitro.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Animals
  • Aqueous Humor / immunology
  • Cattle
  • Cell Count
  • Cell Division
  • Cells, Cultured
  • Dose-Response Relationship, Drug
  • Female
  • Humans
  • Immunoglobulin G / pharmacology
  • Lens, Crystalline / cytology*
  • Male
  • Middle Aged
  • Transforming Growth Factor beta / pharmacology*


  • Immunoglobulin G
  • Transforming Growth Factor beta