Two Molecular Forms of Peptide YY (PYY) Are Abundant in Human Blood: Characterization of a Radioimmunoassay Recognizing PYY 1-36 and PYY 3-36

Regul Pept. 1994 May 5;51(2):151-9. doi: 10.1016/0167-0115(94)90204-6.

Abstract

Two endogenous forms of PYY are abundant in man and dog, PYY 1-36 (PYY-I) and PYY 3-36 (PYY-II). PYY-II is a major molecular form of PYY in human colon, but it is not known, whether PYY-II is also released into the circulating blood. Several radioimmunoassays for measuring PYY-I in plasma have been developed, but it has not been reported, whether they equally detect PYY-II. We characterize a radioimmunoassay for measuring PYY in human plasma which equally recognizes PYY-I and PYY-II. Using this radioimmunoassay and reversed phase HPLC we demonstrate the existence of two forms of PYY in human blood, coeluting with synthetic PYY-I and PYY-II.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adult
  • Animals
  • Chromatography, High Pressure Liquid
  • Cross Reactions
  • Dogs
  • Humans
  • Peptide Fragments
  • Peptide YY
  • Peptides / blood*
  • Radioimmunoassay / methods
  • Reference Values
  • Sensitivity and Specificity

Substances

  • Peptide Fragments
  • Peptides
  • Peptide YY
  • peptide YY (3-36)