Localization of Rabphilin-3A on the synaptic vesicle

Biochem Biophys Res Commun. 1994 Aug 15;202(3):1235-43. doi: 10.1006/bbrc.1994.2063.

Abstract

Rabphilin-3A is a putative target protein for Rab3A small GTP-binding protein which is implicated in neurotransmitter release. Rabphilin-3A is expressed mainly in brain, but its subcellular localization remains to be clarified. Immunohistochemical analysis has revealed that Rabphilin-3A is most abundant in the synaptic area of the rat cerebellum, retina, and neuromuscular junction. Ultrastructural analysis of the neuromuscular junction using the immunogold method indicates that Rabphilin-3A is localized on the synaptic vesicle. Subcellular fractionation analysis of rat brain has shown that Rabphilin-3A is most highly concentrated in the purified synaptic vesicle fraction. These results indicate that Rabphilin-3A is localized on the synaptic vesicle in the presynapse.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptor Proteins, Signal Transducing
  • Animals
  • Cell Fractionation
  • Cerebellum / metabolism
  • GTP-Binding Proteins / metabolism*
  • Immunohistochemistry
  • Male
  • Microscopy, Electron
  • Nerve Tissue Proteins / metabolism*
  • Neuromuscular Junction / metabolism
  • Neuromuscular Junction / ultrastructure
  • Rats
  • Rats, Sprague-Dawley
  • Retina / metabolism
  • Synaptic Vesicles / metabolism*
  • Vesicular Transport Proteins
  • rab GTP-Binding Proteins*

Substances

  • Adaptor Proteins, Signal Transducing
  • Nerve Tissue Proteins
  • Vesicular Transport Proteins
  • rabphilin-3A
  • GTP-Binding Proteins
  • rab GTP-Binding Proteins