High-throughput rapid yeast DNA extraction. Application to yeast artificial chromosomes as polymerase chain reaction templates

Genet Anal Tech Appl. 1994;11(1):7-11. doi: 10.1016/1050-3862(94)90003-5.

Abstract

An automation-assisted method enables DNA extraction of over a thousand yeast colonies in a day by one person. Yeast DNA is extracted essentially quantitatively in three steps: (a) cells are converted into spheroplasts with yeast lytic enzyme, (b) cells are lysed with proteinase K, and (c) DNA is collected by ethanol precipitation. The DNA stored at 4 degrees C is active in polymerase chain reaction experiments for more than a year.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Chromosomes, Artificial, Yeast*
  • DNA, Fungal / isolation & purification*
  • Polymerase Chain Reaction*

Substances

  • DNA, Fungal