Interleukin-1 stimulates de novo synthesis of mitogen-activated protein kinase in glomerular mesangial cells

FEBS Lett. 1994 Aug 15;350(1):135-8. doi: 10.1016/0014-5793(94)00754-3.

Abstract

Interleukin-1 (IL-1) stimulates a time- and concentration-dependent mitogen-activated protein (MAP) kinase activation in rat mesangial cells. A rapid increase in activity (maximal at 10 min) is followed by a second persistent level of activity which steadily increases over 24 h. The second peak of MAP kinase activity is paralleled by a marked de novo synthesis of p42 MAP kinase as measured by immunoprecipitation of [35S]methionine-labelled mesangial cells and by a 60% increase in total p42 MAP kinase protein as detected by Western blot analysis. We propose that IL-1 induced de novo synthesis of p42 MAP kinase is important for the multiplicity of long-term actions of this cytokine in renal mesangial cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Western
  • Cells, Cultured
  • Enzyme Activation
  • Enzyme Induction / drug effects
  • Glomerular Mesangium / cytology
  • Glomerular Mesangium / enzymology*
  • Interleukin-1 / pharmacology*
  • Kinetics
  • Mitogen-Activated Protein Kinase 1
  • Phosphorylation
  • Protein-Serine-Threonine Kinases / biosynthesis*
  • Protein-Serine-Threonine Kinases / metabolism
  • Protein-Tyrosine Kinases / biosynthesis*
  • Protein-Tyrosine Kinases / metabolism
  • Rats

Substances

  • Interleukin-1
  • Protein-Tyrosine Kinases
  • Protein-Serine-Threonine Kinases
  • Mitogen-Activated Protein Kinase 1