Multiple nuclear localization signals of the B-myb gene product

FEBS Lett. 1994 Aug 15;350(1):55-60. doi: 10.1016/0014-5793(94)00733-0.

Abstract

Nuclear entry of the B-myb gene product (B-Myb) is dependent on multiple nuclear localization signals (NLS's). Mutagenesis of the putative NLS's of B-Myb has identified two separate NLS's, NLS1 and NLS2. Each of the two NLS's is essential for efficient nuclear targeting. NLS2 contains two interdependent basic domains separated by 8 intervening spacer amino acids, and both basic domains are required for nuclear entry. Thus, NLS2 belongs to a class of bipartite NLS's. Like the NLS's in yeast transcription factor SW15, NLS2 contains a putative cdc2 kinase site. However, unlike the case of SW15, phosphorylation at this site did not affect the nuclear targeting of B-Myb.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Binding Sites
  • CDC2 Protein Kinase / metabolism
  • Cell Cycle Proteins*
  • Cell Nucleus / metabolism*
  • Cells, Cultured
  • Chlorocebus aethiops
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism*
  • Humans
  • Molecular Sequence Data
  • Protein Sorting Signals / metabolism*
  • Sequence Deletion
  • Trans-Activators*
  • Transcription Factors / genetics
  • Transcription Factors / metabolism*

Substances

  • Cell Cycle Proteins
  • DNA-Binding Proteins
  • MYBL2 protein, human
  • Protein Sorting Signals
  • Trans-Activators
  • Transcription Factors
  • CDC2 Protein Kinase