Evidence for nitric oxide-mediated oxidative damage in chronic inflammation. Nitrotyrosine in serum and synovial fluid from rheumatoid patients

FEBS Lett. 1994 Aug 15;350(1):9-12. doi: 10.1016/0014-5793(94)00722-5.


Reaction of nitric oxide (NO.) with superoxide radical generates peroxy-nitrite, which can decompose to products that nitrate aromatic amino acids. Such nitro-aromatics may be 'markers' of NO.-dependent oxidative damage. Blood serum and synovial fluid from patients with the inflammatory joint disease rheumatoid arthritis contain 3-nitrotyrosine. By contrast, body fluids from normal subjects and patients with osteoarthritis contain no detectable 3-nitrotyrosine; much lower levels were found in serum from patients in the early stages of rheumatoid arthritis. This is evidence that NO. plays a role in joint damage in rheumatoid arthritis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arthritis, Rheumatoid / blood
  • Arthritis, Rheumatoid / metabolism*
  • Chromatography, High Pressure Liquid
  • Chronic Disease
  • Humans
  • Nitric Oxide / metabolism*
  • Spectrophotometry, Ultraviolet
  • Synovial Fluid / metabolism*
  • Tyrosine / analogs & derivatives*
  • Tyrosine / blood
  • Tyrosine / metabolism


  • Nitric Oxide
  • 3-nitrotyrosine
  • Tyrosine