SFE with GC and MS determination of safrole and related allylbenzenes in sassafras teas

J Chromatogr Sci. 1994 Jul;32(7):253-8. doi: 10.1093/chromsci/32.7.253.

Abstract

Safrole (4-allyl-1,2-methylenedioxybenzene), a natural plant component of the aromatic oil of sassafras root bark, possesses carcinogenic and mutagenic activity. Legal restrictions have been placed on safrole as a food additive. However, sassafras teas continue to be accessible from health food establishments in the United States. Supercritical fluid extraction (SFE) with gas chromatographic-mass spectrometric (GC-MS) determination is utilized in the formulation of a rapid, accurate, and specific method for the determination of safrole and related allylbenzenes in unbrewed sassafras teas. Samples are extracted in a static-dynamic mode with CO2 at 690 bar and 80 degrees C with methanol as an extractor-added modifier. Levels of safrole exceeding 10,000 mg/kg (1.0%) are commonly encountered. Lesser amounts of other allylbenzenes, including eugenol and 4-allyl-1,2-dimethoxybenzene, are also reported. Recoveries of safrole and related compounds from previously extracted tea samples fortified at 100 and 1000 mg/kg ranged from 96 to 101%.

MeSH terms

  • Benzene Derivatives / analysis*
  • Beverages / analysis*
  • Chemistry Techniques, Analytical / methods
  • Gas Chromatography-Mass Spectrometry / methods*
  • Safrole / analysis*

Substances

  • Benzene Derivatives
  • Safrole