Recombinant secreted nonpancreatic phospholipase A2 induces a synovitis-like inflammation in the rat air pouch

J Rheumatol. 1994 May;21(5):824-9.

Abstract

Objective: The aim of this study was to test the proinflammatory action of human secreted phospholipase A2 (sPLA2) in an animal model of synovitis-like inflammation and to compare it with a Group 1 (porcine pancreatic) and a Group 2 (Naja mocambique mocambique) PLA2.

Methods: The subcutaneous injection of air in the rat pouch induced the formation of a connective tissue cavity lined with cells closely resembling a synovium. sPLA2 (0.01-10 micrograms/pouch) porcine pancreatic (10 micrograms/pouch), PLA2 or Naja mocambique mocambique PLA2 (10 micrograms/pouch) were injected in a 6-day rat air pouch.

Results: Our data show that recombinant human enzyme PLA2 (0.01-10 micrograms/pouch), but not Naja mocambique mocambique (Group 2 PLA2) and porcine pancreatic PLA2 (Group 1 PLA2), was able to exacerbate the inflammatory response without increasing eicosanoid release.

Conclusion: Only sPLA2 was able to exacerbate rat air pouch and its effect was unrelated to eicosanoid release.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Disease Models, Animal
  • Eicosanoids / analysis
  • Leukocyte Count / drug effects
  • Male
  • Phospholipases A / adverse effects*
  • Phospholipases A2
  • Rats
  • Rats, Wistar
  • Recombinant Proteins / adverse effects
  • Synovitis / blood
  • Synovitis / chemically induced*
  • Synovitis / pathology

Substances

  • Eicosanoids
  • Recombinant Proteins
  • Phospholipases A
  • Phospholipases A2