Stromal keratitis induced by a unique clinical isolate of herpes simplex virus type 1

Ophthalmologica. 1994;208(3):157-60. doi: 10.1159/000310474.


Glycoprotein C (gC)-negative clinical isolates of herpes simplex virus type 1 (HSV-1) are very rare. An HSV-1 strain (TN-1), isolated from a patient with herpetic keratitis, exhibited a gC-negative phenotype. While a gC-negative mutant showed reduced pathogenicity and failed to induce herpetic stromal keratitis (HSK) in a previously reported mouse model, TN-1 induced HSK in mice comparable to RTN-1-20-3, a gC-positive recombinant virus derived from TN-1. Virus growth in eyes and brains and the mortality of TN-1-inoculated mice were equal to or higher than those of RTN-1-20-3-inoculated mice.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Viral / immunology
  • Brain / microbiology
  • Cell Line
  • Corneal Stroma / microbiology*
  • Herpesvirus 1, Human / growth & development*
  • Herpesvirus 1, Human / immunology
  • Humans
  • Hypersensitivity, Delayed / immunology
  • Keratitis, Herpetic / immunology
  • Keratitis, Herpetic / microbiology*
  • Mice
  • Mice, Inbred BALB C
  • T-Lymphocytes, Cytotoxic / immunology
  • Trigeminal Ganglion / microbiology


  • Antibodies, Viral