Isolation and characterization of temperature-sensitive mutants of cyanophage N-1

Acta Virol. 1994 Feb;38(1):11-6.

Abstract

Optimal conditions for the induction of temperature-sensitive (ts) mutants of cyanophage N-1 were established after mutagenesis with N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). A treatment with MNNG (400 micrograms/ml) for 2 hrs at pH 8.0 induced ts-mutants at a maximum frequency of 1.46 x 10(-3). A characterization of 10 such ts-mutants with regard to adsorption, one-step growth and temperature-shift experiments with Nostoc muscorum as host bacterium led to the identification of temperature-sensitive steps in the phage multiplication at the restrictive temperature (37 degrees C). All the mutants were found to be conditionally lethal at 37 degrees C since they resumed growth upon shifting to 28 degrees C.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteriophages / drug effects
  • Bacteriophages / genetics*
  • Bacteriophages / growth & development
  • Bacteriophages / isolation & purification
  • Cyanobacteria / growth & development
  • Methylnitronitrosoguanidine / pharmacology
  • Mutagenesis
  • Mutation*
  • Temperature

Substances

  • Methylnitronitrosoguanidine