Kinetic studies of formaldehyde binding in tissue

Biotech Histochem. 1994 May;69(3):177-9. doi: 10.3109/10520299409106282.


Specimens of rabbit liver were fixed for various periods up to 6 days in buffered 14C-formaldehyde. Binding of the isotope reached a plateau after fixation for approximately 24 hr; the half-maximal binding level was reached after approximately 100 min. Formaldehyde binding at 37 C was faster than at 25 C, and faster at pH 7.0 than at pH 4.0. During rinsing of the fixed tissue in water for up to 26 days there was a progressive decrease in isotope content to 10-20% of the pre-rinse level, indicating that formaldehyde fixation is a reversible process.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Carbon Radioisotopes
  • Formaldehyde / metabolism*
  • Formaldehyde / pharmacokinetics*
  • Hydrogen-Ion Concentration
  • Kinetics
  • Rabbits
  • Temperature
  • Tissue Fixation / methods


  • Carbon Radioisotopes
  • Formaldehyde